Pouring Agar Plates – Edvotek Video Tutorial

Pouring Agar Plates – Edvotek Video Tutorial

September 14, 2019 1 By Ewald Bahringer


Edvotek Instructional videos: Pouring
Agar Plates For this experiment you will need: sterile petri plates, sterile
pipettes and a pipette pump, Edvotek’s ReadyPour Agar and media supplements. Step 1 Break the solid ready pour agar medium into small chunks by squeezing
and shaking the plastic bottle one large One large bottle of ReadyPour will make five large LB source plates and forty small plates. STEP TWO Loosen, but do not remove, the cap
on the ready pour bottle. This allows steam to vent during heating. STEP THREE Microwave the ReadyPour Agar on high for 30 to 60 seconds to start the
melting process Carefully remove the bottle from the
microwave and mix by swirling. Continue to heat the solution in twenty to thirty
second intervals until the agar is completely dissolved. Keep a close eye on
the media to prevent it from boiling over. STEP FOUR Cool the ready pour medium to sixty degrees Celsius with careful swirling to promote even dissipation of heat. STEP FIVE While the medium is cooling label
the petri dishes. Consult your protocol for specific
labeling instructions. STEP SIX Pour 10 mL of the cooled ReadyPour medium
into each of the five large petri dishes using a sterile 10 mL pipette and pipette
pump. step 7 Using a fresh sterile 10 mL pipette pour 5 mL of the medium into small petri plates. These are the control plates. STEP EIGHT Add any media supplements to the
remaining ReadyPour, such as antibiotics as specified in the protocol. Re-cap and
swirl the bottle to mix. Only add reagents to the cooled media. STEP NINE Using a fresh pipette pour 5 ml of the medium into labeled petri plates. STEP TEN Wait at least 20 minutes for the
LB Agar to solidify. For optimal results leave the plates at room
temperature overnight. If the plates need to be stored
they should be inverted and placed in a sealable plastic bag to ensure that they
don’t dry out. The plates should not be stored at room temperature for more than two days.